FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787875

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787875
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116985984
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCT1247390.10662730331866081No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGC1919750.036.307731
TGGCGCG1265500.033.189554
AGGGCGA779600.031.082044
CAATCGC308050.029.9353261
GTGGCGC1545000.029.423053
GGTGGCG2449950.024.2667482
TCAATCG218200.024.1724053
GGGGACC6150850.023.233303145
GGGCGAT264700.023.0933591
CGGAGGG1106150.022.8384971
GGCGATC274200.022.4607732
GGCGCGT1710750.022.3258295
GGGCGAG1155350.021.8517195
GCGAGGC1188350.020.7045047
CGTGCCT1884900.020.5975599
GAGGGCG1216300.020.3167553
AATCGCC460200.019.982472
CGACCGT92050.019.7658371
GGCGAGG1339750.019.0280886
GCGTGCC1978200.018.8817128
Produced by FastQC (version 0.11.5)