FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787876

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787876
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112312603
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1739200.15485350295015424No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1676950.14931093708156687No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1318720.11741513995539753No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1228230.10935816348232975No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1192880.10621069836659382No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1140470.10154425857265546No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA1127820.10041793795839635No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT349150.045.941866
AGACCGC407600.039.116174
CCGCGTT430400.037.4266667
AACGTAT517600.036.6379476
GAACGTA527050.036.297095
CGTATTC546500.034.6778878
CAGACCG481400.034.0046773
GCGTTCT508300.031.5573489
TCGACCG488750.030.657883145
GGGTAGC1029750.030.0943226
TCGCTAT823500.028.1249122
GTAGCTT1163800.026.7021528
CGCGTTC600050.026.6909968
GGTAGCT1196150.025.9799927
ATCGACC226050.025.977104145
AGAACGT493000.025.7320374
CGCTATG914000.025.2483433
CGAGAAC526250.025.0487122
GACCGCG663650.024.356215
AGGGTAG1141500.023.874265
Produced by FastQC (version 0.11.5)