FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787877

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787877
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112312603
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1562760.13914377890431406No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGATC118450.026.9846252
CGGTGGC1597150.025.1995681
CAATCGC216050.023.9367561
TGGCGCG1104950.023.8784434
GTTCGAT139350.023.5838341
TACGCTG380300.022.431166145
GTGGCGC1340950.021.492093
TCAATCG168850.020.9487923
GTTTGAT815200.020.511641
GGCGCGT1149000.020.0867025
GGGGACC3953700.019.272022145
CGCACAA338250.019.0231231
TTTGATC879300.018.742292
TACCGTC222000.017.8031589
GGCGATC230950.017.2661722
GCGACAT284200.016.7435517
ATATCGG227700.015.52961145
AATCGCC340000.015.5033472
GGTGGCG2217600.015.4811222
ACGCCCG858100.015.1688666
Produced by FastQC (version 0.11.5)