FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787878

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787878
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132851377
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA3317510.2497158911646057No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT2827190.2128084829711626No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC1677960.12630354595421317No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1667780.1255372761397874No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT680000.072.977236
AGACCGC711900.068.998924
CCGCGTT731950.068.0853047
CAGACCG780850.063.5840533
TCGACCG979700.060.03929145
GCGTTCT918400.054.1624159
GTGCTCT1448350.052.1027145
CTCGCTA483650.042.701051
TCGCTAT501150.040.4135322
CGCGTTC1241350.039.8754168
CGCTATG535200.037.6855963
GACCGCG1344000.037.0698555
ACCGCTA229150.032.4430162
TCAGACC1655800.031.2021682
CTCAGAC1996650.028.7599121
CTTCGAC1307750.026.597742145
CGATCCT1030950.025.941036145
CCGCTAA325650.024.9926263
GAGTCTT1723450.023.2593944
CGCTAGA489400.021.8356321
Produced by FastQC (version 0.11.5)