FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787879

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787879
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132851377
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCGA949550.039.365694
CAATCGC346500.033.7711831
CGGAGGG1138450.033.398191
TGCGACA530050.032.3939976
GCGACAT543400.031.6713927
TCAATCG224850.031.0015683
GGGCGAT303050.028.4331191
GAGGGCG1426800.026.4865283
TACCGTA221550.026.4593879
CGACATC678700.025.5521628
GCGAGGC1477450.025.354697
GGCGATC342050.025.2169932
GGGCGAG1598450.024.0880645
GCTGCGA731850.023.5851544
GGCGAGG1689400.023.0360036
CTGCGAC774250.022.43355
CTACCGT263850.022.367418
AATCGCC530650.021.992172
ACTACCG283700.020.7325087
CGAGGCC1875850.020.0613218
Produced by FastQC (version 0.11.5)