FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787880

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787880
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114509000
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC1694770.1480032137211922No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1399040.12217729610772954No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1304190.11389410439354111No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1198560.10466950196054457No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1172120.10236051314743819No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCTA581100.037.5762561
CTTCGAC1028150.036.32593145
TCGCTAT617700.035.647082
ACCGCGT349050.034.6074526
CGCTATG655100.033.835153
TCGACCG768000.032.678566145
CCGCGTT370450.032.5189637
ACCGCTA205350.032.1048053
AGACCGC396050.030.3518944
CAGACCG436700.028.1265563
CCGCTAA269550.025.6221124
CGGTGCT620250.025.528923145
GTCTCGC662550.024.7965534
GCGTTCT510200.023.739869
CGCTAAG285650.023.53695
GGGGTCT1307300.021.2374231
GTGCTCT1035400.020.954044145
CGATCCT1159750.020.885572145
GTCTGGA1405150.020.473421
TCTCGCT846300.019.590435
Produced by FastQC (version 0.11.5)