FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787881

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787881
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114509000
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAGGGCGAGGCCCGCGGCGGCGCCGCCGCCGCCGCGCGCTTCCCTCCG1738670.15183697351299896No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATCGC396500.041.429851
GGGCGAT301450.035.795331
AGGGCGA810200.035.2406584
GGCGATC307700.034.921922
TCAATCG247450.034.808133
CGGAGGG914150.032.1949841
AATCGCC527250.030.980432
CGGCCGC2491350.028.10792145
GCGAGGC1081800.026.3528217
GGGCGAG1162750.025.1039315
GGCGAGG1196600.024.5634466
CGGTGGC1636600.024.3663331
ATCGCCC702300.023.3163033
TGGCGCG961250.023.1749944
CGATCTG477600.022.903484
GAGGGCG1341700.021.8756963
GCGACAT475800.021.5139397
TGCGACA473750.021.496446
GGTCACG740400.020.971308145
GGGGACC3699650.020.070534145
Produced by FastQC (version 0.11.5)