FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787883

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787883
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109829113
Sequences flagged as poor quality0
Sequence length35-151
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1717890.15641481143528857No Hit
CGGAGGGCGAGGCCCGCGGCGGCGCCGCCGCCGCCGCGCGCTTCCCTCCG1329650.12106534994960762No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCG233850.036.7134063
AGGGCGA988000.035.1525464
CAATCGC367650.034.8553541
CGGAGGG1208050.029.2648161
GGCGATC286350.028.8405282
GGGCGAT282200.028.8381771
TGCGACA495750.025.546266
GCGACAT522600.024.6026467
AATCGCC529550.024.2632582
GGGCGAG1553800.022.68215
GGCGAGG1666350.021.2244826
GCGAGGC1623050.021.1041287
GAGGGCG1694550.020.9860943
TACCGTA186550.020.883349
ATCAATC464000.020.2279642
GATCAAT448400.020.15651
CGACATC696500.018.4956728
GCTGCGA698950.018.3208474
TTAACGG99750.017.7881073
GGTCACG812800.017.723734145
Produced by FastQC (version 0.11.5)