FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787884

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787884
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences159241815
Sequences flagged as poor quality0
Sequence length35-151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT3404430.21378995209267115No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2904680.1824068634234042No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT2707210.17000622606568508No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA2591650.1627493381684955No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA2581270.16209749932830142No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC2180080.1369037397620719No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT2111280.13258326652456204No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1903030.11950567129619817No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA1890070.11869181470959748No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG1822550.11445172236952963No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1729190.10858894066235053No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC1728700.10855816985004849No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC1719840.10800178332556684No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT672350.049.9434476
AGACCGC669450.049.5510144
CAGACCG741900.045.344463
GTGCTCT1449200.044.927242145
CCGCGTT753350.044.5981257
TCGACCG1451700.039.22611145
GCGTTCT905200.036.8165059
CTCGCTA1247250.035.7269061
TCGCTAT1322700.033.7342072
CGCTATG1443750.030.9441833
CCGCTAA429800.030.1419643
CGCTAAG480500.026.653244
CGATCCT2479850.026.34535145
CTTCGAC1781900.024.8391145
ACCGCTA523600.024.4390352
GAACGTA210500.023.467815
GTCTCGC1124500.022.8053724
GCTATGT1983450.022.6177314
CTATGTT2090400.021.6023455
TCAGACC1671900.021.1733632
Produced by FastQC (version 0.11.5)