FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787885

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787885
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences159241815
Sequences flagged as poor quality0
Sequence length35-151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2781460.17466894609308492No Hit
CCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTA1691790.1062403113152158No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCG263300.028.188853
CAATCGC381950.025.2194371
GCGACAT700900.025.1134437
AGGGCGA1166750.024.7214954
GGCGATC449650.024.506032
TGCGACA746750.023.4467336
TGGCGCG1876400.023.3524234
GGGCGAT470150.023.196041
CGGTGGC3032300.021.4486081
CGACATC920350.019.3817928
GTGGCGC2413800.019.2340283
CGGAGGG1765100.017.1000441
AATCGCC584250.016.6383232
GCTGCGA1080250.016.4552964
CTGCGAC1091500.016.4507945
CTACCGT281100.016.3284938
AGTTACG467750.016.114445145
GGGCGAG1900250.015.9177155
GGGGACC7726550.015.548242145
TACCGTA299600.015.3824179
Produced by FastQC (version 0.11.5)