FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787886

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787886
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101178656
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1547800.15297692825648918No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1349940.13342142042290026No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1148070.11346958394070782No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1115810.11028116443847603No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1105700.10928194183563775No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA1047040.10348427636753744No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT349700.053.4268466
CCGCGTT386950.048.2836047
AGACCGC396700.046.7867554
TCGACCG336000.044.275547145
CAGACCG443650.042.378913
CTCGCTA482100.039.6209951
TCGCTAT524700.036.2794722
GCGTTCT517050.035.934279
CGCTATG564800.033.552353
CGCGTTC585150.031.7852758
GACCGCG646600.028.9549485
GTGCTCT1039500.028.34367145
ACCGCTA129750.027.9046732
CGATCCT1189950.023.343904145
CTTCGAC496950.022.643263145
CCGCTAA187950.021.0543653
TCAGACC1013250.019.7010082
GCTATGT1022000.018.9338384
CTCAGAC1279050.018.4694731
CGCTAAG211000.018.1390254
Produced by FastQC (version 0.11.5)