FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787887

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787887
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101178656
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGC1357650.023.0599841
TGGCGCG855050.022.0750774
CAATCGC195050.021.8444961
TCAATCG141450.021.581633
GTGGCGC1152300.019.8269523
AGGGCGA592550.019.2993414
CGGAGGG695550.016.9028991
GGCGCGT996400.016.84045
GGTGGCG1677500.016.2100832
GGGGACC3353550.016.05711145
TGCGACA278750.015.8541546
ATATCGG204600.015.32144145
CGGCCGC1772600.014.746965145
TACCGTA127300.014.5734529
GCGACAT322550.014.326817
GGCGATC222800.014.0870192
GGGCGAT232350.013.753291
AATCGCC315800.013.6087762
CGTGCCT1275350.013.5155769
GCGTGCC1240650.013.2067838
Produced by FastQC (version 0.11.5)