FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787889

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787889
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145258826
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1746560.1202377885113845No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT468250.024.5163487
TGCGACA466350.024.4013376
AGGGCGA806250.023.3881744
GGGCGAT318400.023.0220261
CGGTGGC1881900.022.2948881
GGCGATC334450.021.8712622
TGGCGCG1115250.021.2725184
CAATCGC248300.021.1635381
CGGAGGG935700.020.673451
TCAATCG184900.019.659093
GGGGACC4899950.018.397335145
TACCGTA206050.018.395659
CGACATC641300.018.150998
GTGGCGC1506950.017.3416253
GCTGCGA684250.016.9244444
GGGCGAG1172250.016.6934435
CTGCGAC699950.016.6869415
CGGCCGC2925600.016.477379145
GAGGGCG1187600.016.0887763
GGCGAGG1228150.015.9639546
Produced by FastQC (version 0.11.5)