FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787893

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787893
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128730280
Sequences flagged as poor quality0
Sequence length35-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1576520.12246691299047902No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCG212200.031.338283
GGGCGAT336850.026.5285191
GGCGATC334200.026.4500372
CAATCGC319450.025.1311531
GCGACAT519950.023.352987
CGGTGGC2179800.021.8251421
AGGGCGA1067900.021.5524644
CGGAGGG1106850.021.0689491
TGCGACA600550.020.4290526
TGGCGCG1470300.020.1202624
TACCGTA184650.017.268779
AATCGCC470150.017.0545462
GTGGCGC1844600.017.0361063
GGGGACC5189650.016.75145
CGACATC753450.016.392128
GGTCACG673050.015.592667145
GCGAGGC1477800.015.1170397
GAGGGCG1563700.014.9673233
GGCGAGG1570750.014.9581276
GGGCGAG1605900.014.8512935
Produced by FastQC (version 0.11.5)