FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787895

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787895
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101295048
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGCG867900.030.66044
AGGGCGA641700.029.1972264
GTGGCGC1142650.025.4542683
TACCGTA155650.024.2831559
CTCGCGT288750.023.8888878
TCGCGTC290150.023.6827079
CAATCGC244100.023.1005781
TGCGACA276650.022.7315046
GCGACAT289750.021.9977867
CGTGCCT1268300.020.0498899
GCGAGGC948650.019.7699187
CGGTGGC1791500.019.7569831
GGGCGAG998250.019.4307485
CTACCGT200850.019.275488
GGCGCGT1322050.018.9924665
CGACCGT83350.018.3342781
GGCGAGG1073150.018.0622656
ACGATAC102400.017.7202665
CGGAACG110300.017.6661241
ACTACCG224400.017.2233927
Produced by FastQC (version 0.11.5)