FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787896

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787896
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100333421
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA2381020.23731075610389085No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1679290.16737095010445222No Hit
GTCCAGAGTCGCCGCCGCCGCCGGCCCCCCGGGTGCCCGGGCCCCCCTCG1506690.15016830732802383No Hit
GGCCCGGCTCGCGTCCAGAGTCGCCGCCGCCGCCGGCCCCCCGGGTGCCC1426430.14216897876929763No Hit
CCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCGCGGCGCACCGCCG1045020.10415472627012289No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1030790.10273645508409406No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT460900.072.013076
AGACCGC504450.065.651654
CCGCGTT505900.065.568147
CAGACCG551250.060.559363
TCGACCG581750.058.10962145
GCGTTCT627150.052.7310379
GTGCTCT1046000.050.56843145
CGCGTTC714750.046.2303478
GACCGCG801400.041.4988335
CTCGCTA301200.040.898351
TCGCTAT319700.037.871262
CGCTATG340050.035.2341843
GTCGGCT304150.032.6943861
TCAGACC1087300.031.958332
CTCAGAC1332600.029.4291341
TTCGGCG488350.029.0988487
ACCGCTA132500.028.6210772
GGATTCG541100.026.6790644
ATTCGGC544400.026.3222586
CGGGATT527200.026.1905632
Produced by FastQC (version 0.11.5)