FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787897

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787897
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100333421
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA335300.031.021896
GCGACAT341400.030.5846527
AGGGCGA629000.026.896094
CAATCGC281200.024.203841
CGACATC478800.022.0858388
TACCGTA140950.021.5765429
GCTGCGA489800.021.5228944
CTCGCGT261100.021.333538
TCGCGTC261100.021.332879
CTGCGAC498450.021.2552625
TGGCGCG629250.020.2010734
TCAATCG208550.018.9547963
ACGATAC100750.018.4951555
GCGAGGC911350.018.452887
GGGCGAG954050.018.3524785
CGGAACG108500.017.4942041
GGCGAGG1004550.017.3304166
AATCGCC394700.017.3244672
AGGGATC617700.016.4379942
CTACCGT187850.016.3672188
Produced by FastQC (version 0.11.5)