FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787898

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787898
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91613348
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1664540.1816918643776669No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1644270.1794793046969531No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1414560.15440544755552435No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1202330.1312396093198122No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC960830.10487882180662145No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT407900.065.677336
CCGCGTT446250.059.989347
AGACCGC451850.059.0154154
TCGACCG611600.057.524952145
CAGACCG470900.057.1397633
GCGTTCT547900.048.7867749
GTGCTCT945150.040.37168145
CGCGTTC689900.038.668948
CTCGCTA432900.038.181191
TCGCTAT445650.036.1258242
GACCGCG765600.035.102485
CGCTATG474850.033.825843
ACCGCTA221700.031.6767482
CCGCTAA242550.030.2645743
TCAGACC985200.028.5261692
CTCAGAC1204750.026.4634081
CGATCCT892300.026.335775145
CGCTAAG273400.026.1824994
CTTCGAC826100.024.460499145
GAGTCTT1082700.022.0063234
Produced by FastQC (version 0.11.5)