FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787899

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787899
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91613348
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT324050.031.424577
TGCGACA327650.030.8000666
CAATCGC226450.026.2393381
AGGGCGA581100.025.0898724
TGGCGCG748800.024.9665744
CGACATC425100.024.0775978
CTGCGAC489400.020.9809075
TCAATCG171050.020.506173
GTGGCGC986450.020.2089043
GCTGCGA509550.020.0495724
GGCGATC255900.018.489142
AATCGCC321000.018.4243412
CGGTGGC1388100.017.8941461
GGGCGAT270650.017.8485241
CTCGCGT161000.017.3235658
GCGAGGC844050.017.2108337
GGGCGAG902750.016.7574045
GGCGCGT1096500.016.44135
TCGCGTC177450.015.7906559
GGCGAGG976050.015.6126296
Produced by FastQC (version 0.11.5)