FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787900

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787900
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111688998
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1865420.16701913647752487No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1817660.16274297670751778No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1408540.12611269016846227No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1326270.11874670054789103No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT435450.063.0975346
CCGCGTT482500.056.9042057
AGACCGC486400.056.305294
TCGACCG546100.052.458168145
CAGACCG537600.051.730683
GCGTTCT627050.043.5972989
CTCGCTA561500.041.1914141
TCGCTAT607000.037.7097742
CGCTATG656000.034.996063
CGCGTTC826450.032.9603928
GTGCTCT1121100.032.954895145
GACCGCG994850.027.7814355
CGATCCT1341250.025.795635145
ACCGCTA204650.025.6545352
TCAGACC1202800.024.5652122
CTCAGAC1488200.022.7618561
CCGCTAA245300.022.4359363
CTTCGAC779750.021.729774145
GCTATGT1148000.020.2692534
CGCTAAG265750.020.1704044
Produced by FastQC (version 0.11.5)