FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787901

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787901
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111688998
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCGA717000.029.8421024
CAATCGC262000.028.901981
TCAATCG178150.028.7422663
TGGCGCG1130950.025.5069184
TGCGACA369350.024.66776
GCGACAT390850.023.5764227
CGGTGGC1640900.023.5122151
CGGAGGG962950.022.9739761
GTGGCGC1453150.021.1063483
GGGCGAG1143850.019.4250265
AATCGCC394250.019.2265342
GCGAGGC1134600.018.8800187
GAGGGCG1178000.018.4997233
GGCGCGT1488650.018.4480325
GGCGAGG1251100.017.8429266
CGACATC532450.017.7576648
GGCGATC242350.017.0847052
CGTGCCT1642100.016.8983089
GGGCGAT239050.016.7359121
GGGGACC4195250.016.7351145
Produced by FastQC (version 0.11.5)