FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787902

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787902
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121265607
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1961730.16177134214155214No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1833510.15119785777347405No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1615240.1331985251185029No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT426500.065.768246
TCGACCG742650.061.757114145
CCGCGTT456200.061.443817
AGACCGC475600.058.5604864
CAGACCG515850.054.408173
GCGTTCT635800.043.6354569
CTCGCTA483800.043.0946771
GTGCTCT1197900.039.341717145
TCGCTAT529700.039.0050132
CGCGTTC719850.038.459288
CGCTATG579200.035.5016863
GACCGCG931800.030.2286245
ACCGCTA166800.029.9136372
CGATCCT1099550.027.907185145
TCAGACC1166050.025.5683442
CTCAGAC1476950.023.7789231
CTTCGAC1006700.023.635094145
CCGCTAA229250.023.521913
TCGCGTC698400.023.3994799
CTCGCGT703950.023.0950268
Produced by FastQC (version 0.11.5)