FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787903

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787903
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121265607
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT428650.030.0460057
TGCGACA433300.029.738526
AGGGCGA704900.027.8634034
CAATCGC264500.023.6604231
CGACATC577100.022.7104728
TACCGTA197350.022.6625149
TGGCGCG975750.022.009084
GCTGCGA620350.021.2118384
GGGCGAT289800.020.5253311
CTGCGAC659300.019.9081025
TCAATCG196800.019.4662113
GGCGATC308750.019.3992122
CGGTGGC1622400.018.3722551
GGGCGAG1115400.018.3049185
CTACCGT246500.018.3015738
GCGAGGC1081850.018.1957267
GTGGCGC1270950.017.3969173
CTCGCGT190350.017.2643898
GGCGAGG1192900.017.2299446
TCGCGTC188400.017.202259
Produced by FastQC (version 0.11.5)