FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787905

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787905
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144584614
Sequences flagged as poor quality0
Sequence length35-151
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAGGGCGAGGCCCGCGGCGGCGCCGCCGCCGCCGCGCGCTTCCCTCCG1836840.1270425634639105No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCGA1134500.037.3461274
TGCGACA527900.025.7667146
GCGACAT539750.025.2927387
GGGCGAG1788400.024.30185
CAATCGC376600.023.617941
TGGCGCG1595350.023.3923854
GGGCGAT381200.023.3494531
TCAATCG270200.023.0652453
GCGAGGC1884850.022.5921577
GGCGAGG1936400.022.5851526
CGGAGGG2005350.022.5599271
TCGCGTC307750.022.4415689
CTCGCGT309600.022.287488
TACCGTA189300.021.772629
GGCGATC424850.020.685532
GAGGGCG2225950.020.0017953
CGACATC695750.019.9598248
CGGTGGC2592750.019.5392341
GTGGCGC2031450.019.4125963
CGAGGCC2387700.018.1454338
Produced by FastQC (version 0.11.5)