FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787907

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787907
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137681534
Sequences flagged as poor quality0
Sequence length35-151
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAGGGCGAGGCCCGCGGCGGCGCCGCCGCCGCCGCGCGCTTCCCTCCG1988990.14446309117967848No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCG296000.032.1191643
AGGGCGA1058650.031.7905984
TGCGACA597950.029.4568986
GCGACAT613800.028.9312887
CAATCGC441300.028.921161
GGGCGAT345550.026.6650371
GGCGATC374200.024.2649752
CGGAGGG1489750.023.2763731
TACCGTA215450.022.3881849
TGGCGCG1575700.022.0438484
CGACATC838200.021.2679678
GCTGCGA864750.020.6241864
CGGTGGC2429600.020.078071
AATCGCC643500.019.9870932
CTGCGAC926800.019.3840895
GGGCGAG1822550.019.0879295
GATCAAT524650.018.866461
GGCGAGG1877900.018.5786156
GAGGGCG1922950.018.2393533
GCGAGGC1852250.018.056997
Produced by FastQC (version 0.11.5)