FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787909

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787909
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97663895
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA297700.024.9088046
GCGACAT303100.024.6344227
TACCGTA156700.023.352889
CTACCGT190600.019.2335578
AGGGCGA491200.018.9843434
CGACATC412550.018.3477828
GGGCGAT209800.017.0347671
ACTACCG218300.016.9107447
GCTGCGA451850.016.8542944
CGGTGGC980800.015.9810081
CTGCGAC488600.015.5447325
CGGAGGG637850.015.477481
GGCGATC230600.015.4447722
TGGCGCG603700.015.3614294
CGGCCGC1331900.014.497502145
GGCGCGT646400.013.8953125
GGGGACC1947450.013.557243145
CGATCTG285000.013.5072974
GCGAGGC693700.013.3272157
GGGCGAG738900.012.9378175
Produced by FastQC (version 0.11.5)