FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787911

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787911
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106488675
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCG237150.027.747473
CAATCGC330500.026.269921
ACTACCG198350.025.99437
AGGGCGA717250.025.4534244
TACCGTA215250.024.8213679
CTACCGT233050.022.9261258
GCGACAT319150.021.566477
TGCGACA313500.021.5375216
CGGAGGG959550.019.6331561
TGGCGCG1115000.019.5564774
CGACCGT112850.019.0931531
GATCAAT384600.017.7820781
CGGTGGC1875700.017.315581
GGGCGAT248650.016.973021
AATCGCC519100.016.9636462
GACCGTT127800.016.7364312
GGCGATC260650.016.532142
GGGCGAG1136000.016.4262225
ACCGTTG133550.016.2061423
GTGGCGC1430550.015.9490453
Produced by FastQC (version 0.11.5)