FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787912

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787912
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107512414
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1850630.17213175029257552No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1473460.1370502200797017No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1180690.10981894611723629No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT575050.063.6150446
AGACCGC614450.059.2352334
CCGCGTT619300.059.1206787
CAGACCG657750.055.8709833
GTGCTCT1171100.051.96415145
GCGTTCT720950.050.662239
TCGACCG466100.048.259068145
CTCGCTA394250.040.50041
CGCGTTC951500.038.3003238
ACCGCTA180550.037.7341462
TCGCTAT422800.037.1395382
CGCTATG462600.033.9715923
GACCGCG1104150.033.2972455
TCAGACC1264750.030.2679632
CTCAGAC1518100.027.9172521
GTCGCGT72550.026.4638561
CCGCTAA291950.025.3744813
CGATCCT894800.024.565365145
CGCTAAG337950.021.4912534
CTTCGAC631400.021.102455145
Produced by FastQC (version 0.11.5)