FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787913

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787913
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107512414
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCG190800.036.9446953
CAATCGC286850.032.3275381
AGGGCGA795400.031.366294
CGGAGGG1009450.025.4198341
TGCGACA304350.023.1791296
GCGACAT311700.022.7756397
AATCGCC432800.021.4378662
GCGAGGC1186000.020.665547
GGGCGAG1230850.020.5340675
GGCGAGG1265850.020.0919576
GAGGGCG1360100.018.8595543
TGGCGCG862850.016.9597344
ATCAATC469950.016.5929642
CGGCCGC3324300.016.518976145
AGGGATC684600.016.2094082
CGAGGCC1544900.016.1162728
CGACATC451850.015.9369688
GGCGATC254600.015.6648432
GGGCGAT255050.015.3314561
GATCAAT498850.015.2612311
Produced by FastQC (version 0.11.5)