FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787915

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787915
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119656864
Sequences flagged as poor quality0
Sequence length35-151
%GC55

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1470060.12285630350466147No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATCGC374850.028.0128961
AGGGCGA1094300.027.746244
TCAATCG248200.026.8563693
TGCGACA556800.025.6843416
GCGACAT557800.025.5596857
GGCGATC358000.023.8909342
TGGCGCG1506750.023.655314
CGGTGGC2320700.023.2402361
GGGCGAT379000.022.7471451
GTGGCGC1882600.021.204323
CGGAGGG1578950.020.2152371
AATCGCC490150.019.2534832
CGACATC761900.019.0581468
CTCGCGT231550.018.5395158
GCTGCGA782250.018.461384
GGGCGAG1726000.018.3250165
TCGCGTC235400.018.1028849
GGCGAGG1774000.017.7620756
CTGCGAC834600.017.4730955
GCGAGGC1727600.017.3286487
Produced by FastQC (version 0.11.5)