FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787916

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787916
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127770508
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA2796220.21884705976124005No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2091340.16367939931803355No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1924890.15065213640693986No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1394750.1091605583974042No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1391420.10889993487385995No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT664150.055.890856
AGACCGC688650.053.643274
GTGCTCT1404800.053.15025145
CCGCGTT714650.051.8647167
CAGACCG737550.050.4369623
GCGTTCT877800.042.0077939
TCGACCG717950.039.956726145
CTCGCTA634400.039.6118051
TCGCTAT694750.035.8642962
ACCGCTA227550.034.004192
CGCGTTC1095550.033.731378
CGCTATG743850.033.480433
GACCGCG1219650.030.5659225
CCGCTAA308550.027.031043
TCAGACC1503550.025.9480932
CGATCCT1330700.024.935999145
CGGTGCT1011050.024.113256145
CTCAGAC1804650.023.8077981
CGCTAAG346600.023.7441164
CTTCGAC957300.023.74168145
Produced by FastQC (version 0.11.5)