FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787917

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787917
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127770508
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1979570.15493168423498793No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA216800.024.5415769
AGGGCGA808900.024.2350644
CAATCGC281400.023.4069581
TGCGACA410850.023.096816
GCGACAT429450.022.1253177
CTACCGT252000.021.6055368
TGGCGCG1055500.021.4736824
TCAATCG200450.021.3654353
GGGCGAT290750.021.2151741
CGGTGGC1747150.021.059631
CGGAGGG972450.020.874091
GGCGATC302400.020.289982
GTGGCGC1378900.017.8532093
ACTACCG309250.017.6458637
CGACATC565400.017.101098
GGGGACC4050850.016.85038145
CGTTCTT760300.016.164759145
GGGCGAG1291200.015.7250415
GAGGGCG1284050.015.6098183
GCTGCGA624400.015.4052454
Produced by FastQC (version 0.11.5)