FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787918

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787918
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113183708
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1745090.15418208422717516No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1692720.14955509321182514No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1507050.13315078880433923No Hit
CCCCCGGGTGCCCGGGCCCCCCTCGCGGGGGACCGTGCCCCCGCCGCCGG1196430.10570690969057137No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT451750.051.097326
AGACCGC479950.047.850644
TCGACCG450900.041.427498145
CAGACCG575900.040.3730133
GCGTTCT591400.039.031689
GTGCTCT1196600.038.017223145
CCGCGTT614050.037.783397
ATCGACC238450.034.272808145
GAACGTA383400.033.3266075
AACGTAT390700.032.7667926
TCGCTAT565300.032.1470832
CGTATTC428250.029.7492798
CGCGTTC804650.028.6181768
CGCTATG667500.027.1869413
CTCGCTA713850.027.034261
GGAACGT392550.026.8539734
GACCGCG897100.025.834915
CGGAGTA445750.024.7327634
CGATCCT1241950.024.424747145
GAGTACC451150.024.0244796
Produced by FastQC (version 0.11.5)