FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787921

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787921
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127070989
Sequences flagged as poor quality0
Sequence length35-151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAGGGCGAGGCCCGCGGCGGCGCCGCCGCCGCCGCGCGCTTCCCTCCG1570810.12361672891363111No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1501380.11815285391380718No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCGA1043100.034.898484
CAATCGC373800.028.5399511
TCAATCG251750.027.8120443
CGGAGGG1354150.027.6753311
TGCGACA482800.025.113126
TACCGTA273800.025.1009549
GGCGATC301450.024.1693422
GCGACAT516050.023.9354217
CTACCGT299700.022.9524658
GGGCGAG1752150.021.397555
GGGCGAT343050.021.2978781
GGCGAGG1838600.020.3814286
GAGGGCG1896650.019.7598023
GCGAGGC1906900.018.95897
CGGTGGC2235000.018.7624911
AATCGCC584550.018.4858572
GGGGACC4595900.017.830568145
CGACATC711500.017.6442858
CGGCCGC5933850.017.273897145
ACTACCG424050.016.4109467
Produced by FastQC (version 0.11.5)