FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787922

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787922
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117178378
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1699110.14500200711090233No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1429750.12201483109793515No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT324050.041.77726
CCGCGTT355800.038.152547
ACCGCTA224500.038.014322
AGACCGC351950.037.9262544
CTCGCTA566250.036.481671
CAGACCG392650.034.7130053
TCGACCG592700.033.81031145
TCGCTAT622300.033.260362
CGCTATG682300.030.3601843
GCGTTCT465050.029.0448829
CCGCTAA316750.028.9839943
GGTGCTC1048750.025.046251145
CGCTAAG363350.024.9294284
CGATCCT1157150.024.750275145
CTTCGAC768950.024.435347145
CACCGCT442400.020.5216161
CGCTAGA310200.019.4517841
CGCGTTC696200.019.2518568
TCGTACG288200.019.05337510-14
GCTATGT1115350.018.8306794
Produced by FastQC (version 0.11.5)