FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787923

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787923
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117178378
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1334470.11388363815720338No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCG170800.027.6336353
CAATCGC244400.026.8129231
AGGGCGA737850.021.406244
TGCGACA317850.019.3962556
GCGACAT350700.017.842577
AATCGCC367900.017.8057332
TGGCGCG976400.017.7706784
CGGAGGG979100.016.9618761
CGGTGGC1728650.016.8567141
ACGCCCG882100.016.7404026
GGGCGAT263650.016.1440581
GGGGACC3868350.016.106396145
CTCGCGT142950.015.9253058
GGCGATC269600.015.9169882
TCGCGTC150800.015.1779039
GGGCGAG1097750.014.9090725
GGTCACG646050.014.812605145
CGGCCGC3357100.014.593996145
GTGGCGC1353550.014.4662633
CCCCACG1030550.014.1229822
Produced by FastQC (version 0.11.5)