FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787924

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787924
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126405734
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT2859970.22625318563475927No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1973640.1561353221523954No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC1905930.1507787613495445No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1646870.1302844378879205No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1360930.1076636286135564No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT477450.053.318766
TCGACCG1133650.052.21788145
AGACCGC511850.049.3700074
CCGCGTT522200.048.7495967
CTCGCTA440750.040.90831
CAGACCG631700.040.5268973
GCGTTCT664400.038.297079
TCGCTAT486500.037.1189842
GTGCTCT1282950.036.368637145
CGCTATG530650.033.772193
CGCGTTC830900.030.4503638
CTTCGAC1393700.027.386415145
GACCGCG935150.027.362395
ACCGCTA175200.027.3604132
TCGCGTC404300.025.4391359
CTCGCGT405200.025.2903338
CGATCCT1128650.025.117268145
GAGTCTT1786100.022.2622474
TCAGACC1281200.021.0718022
GGAGTCT1897900.021.0037483
Produced by FastQC (version 0.11.5)