FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787925

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787925
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126405734
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA573550.029.4706866
GCGACAT598150.028.510217
GGGCGAT317450.026.389411
GGCGATC332350.025.3018512
CGACATC733850.023.3651268
AGGGCGA810250.022.044034
GCTGCGA780150.021.7373544
CTGCGAC809850.021.1890685
TGGCGCG1021650.018.5933674
CGGTGGC1562850.018.4400271
CAATCGC275550.018.3897171
CGGAGGG1039950.018.0711121
TACCGTA192500.017.2607489
TCGCGTC162550.016.854239
CGATCTG533450.016.3944954
CTCGCGT171750.016.316578
ACGCCCG879250.015.5947376
GGGGACC3550750.015.421252145
TCAATCG208300.015.2894553
GCGAGGC1161450.015.2874277
Produced by FastQC (version 0.11.5)