FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787927

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787927
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124674061
Sequences flagged as poor quality0
Sequence length35-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1943870.15591615324056862No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCG233350.032.8205763
CAATCGC341150.030.476291
AGGGCGA749550.027.6216724
GCGACAT423700.024.3328327
TGCGACA426100.024.0070156
TGGCGCG1332100.021.4821324
AATCGCC505350.020.7686182
CGGTGGC2207850.020.4353431
GGCGATC291850.018.4157852
GTGGCGC1741500.018.1349413
CGACATC575300.018.0606128
CGGAGGG1200950.018.0221711
GGGCGAT300500.017.548991
GATCAAT468150.017.23041
TACCGTA167800.017.0382969
GGGGACC5105150.016.952496145
GGGCGAG1263150.016.7551365
ATATCGG222100.016.401339145
ATCAATC521300.016.2847062
CTGCGAC672850.015.6077555
Produced by FastQC (version 0.11.5)