FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787928

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787928
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112617706
Sequences flagged as poor quality0
Sequence length35-151
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT2347630.2084601154990673No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1622450.14406704395133035No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC1582710.14053829155426056No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1402420.12452926363106705No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC1392480.12364663155188048No Hit
CCACACACGACCGGTCGGAGGCAGAACGGCAGCCCCTCGGCGGCCGGCCG1282660.11389505660859404No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1275150.11322819877009392No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1273690.11309855663371442No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA1227520.10899884606067184No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG1206200.10710571568559567No Hit
CACCGCTAAGAGTCGTACGAGGTCGATTTGGCGAGGGCGCTCCCGACGAC1183010.1050465368207731No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1169190.10381937632435881No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCTA339150.047.800942
CCGCTAA452550.038.5899053
CTCGCTA822150.038.035051
ACCGCGT229850.034.7915736
TCGCTAT902600.034.6890532
TCGACCG658500.033.726936145
CGCTAAG535600.032.414164
CGCTATG997400.031.5557083
CCGCGTT261450.030.4708257
AGACCGC257150.029.7340494
CACCGCT572250.028.7808421
CAGACCG280700.028.1445473
GTCGCGT140200.027.0620441
CGATCCT1498500.026.704409145
GTCTCGC768900.024.8448144
AGAGTCG715600.024.5728349
GCTATGT1397050.022.6672174
CTTCGAC837650.022.267248145
CGCTAGA544750.021.8216691
CGTATCG159950.021.7458843
Produced by FastQC (version 0.11.5)