FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787929

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787929
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112617706
Sequences flagged as poor quality0
Sequence length35-151
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAGGGCGAGGCCCGCGGCGGCGCCGCCGCCGCCGCGCGCTTCCCTCCG1746600.1550910653427801No Hit
CCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTA1259760.11186162857907975No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1193950.10601796488378123No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCG222650.036.343
AGGGCGA843750.033.1079254
CAATCGC334950.031.4583241
CGGAGGG1198700.024.1004941
AATCGCC490350.021.8605162
GATCAAT394600.021.3401781
TGCGACA341300.021.1538316
TACCGTA184050.021.0836669
GGGCGAG1365350.020.6784825
GGCGATC254800.020.4364832
TGGCGCG1200900.019.6848134
GGGCGAT280550.018.8903751
GCGACAT390400.018.7105227
ATCAATC472800.018.3645482
GGCGAGG1546900.018.350066
CTACCGT213850.018.1741458
GAGGGCG1614350.017.9306833
GCGAGGC1573850.017.5700657
CGGCCGC6030950.016.950129145
GTGGCGC1580400.016.6427293
Produced by FastQC (version 0.11.5)