FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787930

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787930
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157684530
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT2278470.1444954682618517No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT2261280.14340531693248537No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2177790.1381105679802578No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG872150.054.298367145
ACCGCGT394050.052.5245326
CCGCGTT436600.047.2898867
CTCGCTA580350.044.231741
AGACCGC471800.043.6144334
TCGCTAT646300.039.2321472
CAGACCG536250.039.149753
CGCTATG703500.035.839483
GCGTTCT686650.030.032049
ACCGCTA244300.029.3733652
CGATCCT1497100.026.753716145
CGCGTTC777150.026.3153468
CCGGTAA209200.025.1945341
GACCGCG887100.023.4666845
CTTCGAC1258550.023.135527145
TCGCGTC310950.022.7288069
CCGCTAA346750.021.8939083
CTCGCGT333750.021.2517938
CGCTAGA343550.020.2512591
CGGTAAT236650.020.2063672
Produced by FastQC (version 0.11.5)