FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787931

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787931
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157684530
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2134300.1353525295093945No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA306050.030.6833469
TGCGACA501250.027.1718926
CTACCGT358000.026.4433698
GCGACAT531500.025.7924427
AGGGCGA866450.024.2226944
ACTACCG407700.023.717517
TGGCGCG1266250.022.338334
GGGCGAT328250.021.6481951
GGCGATC352000.020.338352
CAATCGC361400.019.7333351
CGGTGGC1944500.019.6021791
CGACATC708900.019.4363528
CGGAGGG1129700.019.278561
GTGGCGC1631050.018.4073243
GCTGCGA747900.018.3294774
CGACCGT149600.017.1171671
GGCGCGT1522450.017.0008245
CTGCGAC844750.016.5808775
GGGCGAG1305350.016.2532025
GGGGACC4517900.016.188225145
Produced by FastQC (version 0.11.5)