FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787932

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787932
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158282627
Sequences flagged as poor quality0
Sequence length35-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT4029020.2545459395237356No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA2835860.17916432483774736No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT2664740.16835328364874813No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA2602530.1644229723329017No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG2454060.15504291573325985No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT2453670.15501827626350934No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA2372770.1499071657434647No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2361880.14921915593427698No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC2092210.132181910273703No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG2070340.13080020462384667No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC1971530.12455757383910492No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC1900620.1200776128134391No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG1811340.11443706958439602No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT1626860.10278196861112243No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA1622360.10249766703707792No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT658450.051.5017476
AGACCGC708550.047.42864
CCGCGTT722350.046.945847
CAGACCG751750.045.084773
GCGTTCT891550.037.8403789
CTCGCTA1459300.036.8914451
ACCGCTA339950.033.8556942
TCGCTAT1647500.033.0124662
GTGCTCT1439500.031.308504145
CGCTATG1801700.030.3178163
TCGACCG524700.030.243292145
CCGCTAA427750.029.2077123
GTCTCGC1306900.027.7724
CGCGTTC1253000.026.825048
CGATCCT3127450.025.85289145
CGCTAAG483600.025.4475364
GGGGTCT2067400.023.4051131
GCTATGT2358550.023.2551694
TCAGACC1559050.022.7391452
TCTCGCT1616650.022.497215
Produced by FastQC (version 0.11.5)