FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787933

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787933
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158282627
Sequences flagged as poor quality0
Sequence length35-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2287050.1444915366485546No Hit
CCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTA1938760.12248722659878523No Hit
CTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCT1726320.10906566517878175No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG1720630.10870618163293436No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCG263200.034.612893
CAATCGC386050.031.6714171
TGGCGCG1990050.027.9335964
CGGTGGC3305900.026.9163861
GTGGCGC2540500.023.7137153
AGGGCGA995500.023.3320184
AATCGCC597350.020.556632
CGTGCCT3171200.018.4850989
GGGGACC8566500.018.423813145
GGCGCGT2882100.018.0600415
ATCAATC607900.016.4530372
GCGTGCC3157600.016.3213718
GATCAAT605550.016.3098451
CGGAGGG1519300.016.0285261
GGTGGCG4336000.015.672172
TGCGACA348700.015.6360436
GGGCGAG1586100.015.0634835
GGCGAGG1678400.014.3173416
GCGACAT395050.014.26209457
ATATCGG264850.014.24004145
Produced by FastQC (version 0.11.5)