FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787934

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787934
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116236698
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1703780.14657849279235377No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1621330.13948520801924363No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1348080.11597714174571615No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1336020.11493960366974637No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT327300.054.303456
TCGACCG554150.050.45587145
CCGCGTT362600.049.0516787
AGACCGC383600.045.9050144
CAGACCG422950.042.126423
CTCGCTA468000.040.105511
ACCGCTA236000.038.312282
TCGCTAT496250.037.012252
CGCTATG531100.034.557823
GCGTTCT528900.033.4977269
CCGCTAA295600.032.4000933
GTGCTCT1076600.031.698017145
CGCTAAG347500.027.070844
CGATCCT1172050.026.419985145
CGCGTTC724500.024.2969868
CGCTAGA375700.022.9428631
CTTCGAC759600.022.156878145
CACCGCT465200.020.6942331
AGAGTCG472050.019.9956469
GACCGCG908250.019.7426345
Produced by FastQC (version 0.11.5)