FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787935

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787935
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116236698
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1607260.13827474693061223No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCGA699700.029.3158844
GCGACAT342700.024.3149577
CAATCGC232550.024.0508331
TGCGACA347450.023.6001386
CGGAGGG923750.022.9621
TCAATCG169100.022.1877333
GGCGATC239900.019.85962
GGGCGAG1108150.019.1613125
CGGCCGC3329950.019.0991145
GGGCGAT240100.018.9401781
CGGTGGC1663250.018.8458081
GGGGACC3500400.018.550514145
GAGGGCG1135050.018.417343
CGACATC458700.018.2349518
GGCGAGG1205600.017.6177316
GCGAGGC1162000.017.5927077
GCTGCGA517550.016.149254
AATCGCC358100.015.601352
CTGCGAC536400.015.5581485
TGGCGCG899600.015.2901573
Produced by FastQC (version 0.11.5)