FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787936

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787936
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165611988
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT3205470.19355301743011502No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT2544110.1536187102590665No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC1930600.11657368668263315No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1705170.10296174936321638No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG1116900.060.333786145
CTCGCTA673000.040.8031161
ACCGCGT273300.039.150676
TCGCTAT734700.037.0287062
ACCGCTA237150.035.222092
CGCTATG787700.034.5008853
CCGCGTT312150.034.2365267
AGACCGC340050.031.0712264
CAGACCG409850.026.7963543
CTTCGAC1477900.026.61146145
CGATCCT1717100.026.367115145
CCGCTAA351400.025.4678633
GAGTCTT2052800.022.0236634
CGCTAAG409950.021.4097464
GTCGCGT96050.021.3617361
TCGCGTC847650.020.8814989
CTCGCGT847450.020.8332128
CGCTAGA520350.020.7818981
AGTCTTG2270750.020.2555145
GTCTTGG2301150.020.1314076
Produced by FastQC (version 0.11.5)