FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787937

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787937
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165611988
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAGGGCGAGGCCCGCGGCGGCGCCGCCGCCGCCGCGCGCTTCCCTCCG1897340.11456537796044088No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCGA1014250.029.9085754
TGCGACA627950.029.4049916
GCGACAT646400.028.8158267
TCAATCG295500.025.1129043
GGGCGAT395100.024.4812181
TGGCGCG1437650.023.5260834
CGACATC807600.023.2716358
GGCGATC438350.022.2049392
CAATCGC403950.021.4902341
GCTGCGA872750.021.2897344
CTGCGAC902000.020.7575875
GGTCACG727850.019.76706145
CGGTGGC2334700.019.544681
TACCGTA233650.018.8459369
GGGCGAG1707100.018.5357935
CGGAGGG1744600.018.5147691
GTGGCGC1906700.018.4268423
GCGAGGC1727550.017.6922977
GAGGGCG1792650.017.5708053
GGCGAGG1885450.016.9534456
Produced by FastQC (version 0.11.5)