FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787938

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787938
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106361005
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT2011170.18908903690784043No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1454980.13679637570179032No Hit
GTCCAGAGTCGCCGCCGCCGCCGGCCCCCCGGGTGCCCGGGCCCCCCTCG1128270.10607929099579305No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1111380.10449130299210693No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC1103540.10375419073935979No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1085600.10206748234468073No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG732950.059.12609145
ACCGCGT254150.056.8348926
CCGCGTT276450.052.181447
AGACCGC334550.043.1240544
CAGACCG352400.041.6112673
CTCGCTA368500.041.15481
CGCGTTC352750.040.8763548
TCGCTAT397550.037.656782
GCGTTCT402750.035.929289
GACCGCG421850.034.3821225
CGCTATG433200.034.2653123
GTGCTCT960600.027.73279145
CTTCGAC918100.025.80958145
CGATCCT999650.025.379583145
TTCGGCG223950.025.3526927
GTCGGCT259400.023.9147931
ACCGCTA105250.021.5158822
TCGGCGA262550.021.3807188
GAGTCTT1339250.021.2669334
GGATTCG295450.020.0672824
Produced by FastQC (version 0.11.5)